Amplification factor = 10^(−1/slope); percent efficiency = (factor − 1) × 100.
How to use qPCR Efficiency Calculator
- Read the method and enter quantities in the units shown on the form.
- Check the measurement basis, recorded event, genetic assumptions or the externally supplied care-plan instruction before calculating.
- Select Calculate, then read both the result and its limitations. Reset clears the result and restores the starting form.
Method and scope
Amplification factor = 10^(−1/slope); percent efficiency = (factor − 1) × 100.
Work through the input issue
For a related perspective, read qPCR Standard-Curve Slopes: Check the Log Base and Apparent Efficiency.
Related measurement
Annealing Temperature Calculator can help with a related question, using its own stated assumptions.
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