Insert mass = vector mass × insert length ÷ vector length × chosen insert-to-vector molar ratio.
How to use Ligation Calculator
- Read the method and enter quantities in the units shown on the form.
- Check the measurement basis, recorded event, genetic assumptions or the externally supplied care-plan instruction before calculating.
- Select Calculate, then read both the result and its limitations. Reset clears the result and restores the starting form.
Method and scope
Insert mass = vector mass × insert length ÷ vector length × chosen insert-to-vector molar ratio.
Work through the input issue
For a related perspective, read Molar Ratios in Ligation: Equal DNA Mass Does Not Mean Equal Molecule Count.
Related measurement
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